All kinds of plant tissue culture concepts
simple understanding of full plant tissue culture
Ratings 4.24 / 5.00
What You Will Learn!
- plant tissue culture introduction
- steps of plant tissue culture
- callus culture stages steps
- cell suspention culture
- batch culture types
- continuous culture
- open continuous culture
- closed continuous culture
- Difference between Batch and continuous culture
- Meristem culture
- meristem culture steps
- meristem culture application
- Protoplast culture
- Fusion of protoplast
- Application of protoplast
- Bud culture
- Axillary bud culture
- seed culture
- Embryo culture
- Embryo culture applications
- Anther culture
- Factors of anther culture
- Anther culture applications
- Anther culture limitations
- Plant tissue culture media preparation and growth regulators
- Major types of Media
- MS media
- B5 media
- N6 Media
- Nitches media
- Classification of Plant tissue culture
- inorganic nutrients
- Carbon Energy sources
- Macro nutrients
- Micro nutrients
- Difference between macro and micro nutrients
- Organic supplements
- Vitamins
- Amino acids
- other organic supplements
- activated charcoal
- Antibiotics
- Growth regulators
- Auxins
- Cytokinin
- Gibberellins
- Plant growth promoters
- Plant growth functions
- Plant growth applications
- commercial usage
- plant growth inhibitors
- ABA
- applications of ABA
- effects of ABA
- Ethaline uses
- Solidifying agents
- Gelatin
- PH of Medium
- Suitable, selection and sterilization of medium
- Overview
Description
Who is the father of tissue culture?
Gottlieb Haberlandt
Gottlieb Haberlandt is known as the father of plant tissue culture.
Gottlieb Haberlandt (28 November 1854 to 30 January 1945) was an Austrian botanist.
His original idea presented in 1902 was called Totipotentiality.
Explant
An explant is the part of a plant which has got the regeneration potential and is capable to give rise to the whole plant.
Step 1 Explant for callus culture
Step 2 Surface sterilization
Step 3 Factors affecting callus culture
Physical factors
Growth regulators
Step 4 Suspension culture from callus
Callus culture callus is the undifferentiated and unorganized mass of plant cells
It is basically a tumor tissue which usually forms on wounds of differentiated tissues or organs
Callus formation in vivo is frequently observed as a result of wounds at edges of stems or roots
Explant for callus culture
The explant for callus culture may be the differentiated tissue from any part of the plant
The explant for callus culture may be the differentiated tissue from any part of the plant
The selected explant tissues may be at different stages of cell division cell proliferation and organization into different distinct specialized structures
If the explant used possesses meristematic cells then the cell division and multiplication will be rapid
Factors affecting callus culture
Many factors are known to influence callus formation in invitro cultures
MS medium most commonly used physical factors and growth factors
Rest will continue with live session.
Major types of Media
MS media
B5 media
N6 Media
Niches media
Classification of Plant tissue culture
inorganic nutrients
Carbon Energy sources
Macro nutrients
Micro nutrients
Difference between macro and micro nutrients
Organic supplements
Vitamins
Amino acids
other organic supplements
activated charcoal
Antibiotics
Growth regulators
Auxins
Cytokinin
Gibberellins
Plant growth promoters
Plant growth functions
Plant growth applications
commercial usage
plant growth inhibitors
ABA
applications of ABA
effects of ABA
Ethaline uses
Solidifying agents
Gelatin
PH of Medium
Suitable, selection and sterilization of medium
Overview
Who Should Attend!
- Biotechnology
- Micobiology
- Agricultural biotech
- Btech biotech
- Bsc biotech
- plant biotech
- Msc biotech
- BSc agriculture
- All life science students
- Botany
- Biochemistry
