Analytical Techniques
Qualitative and Quantitative techniques for analysis of various compounds
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What You Will Learn!
- This course deals with the analytical techniques routinely used in Biochemistry
- Understanding the strong basis of the experiments and techniques on which the theories are built.
- This course deals with various techniques their principles, experimental details
- It Include general Laboratory techniques, separation techniques based on hydrodynamic properties, centrifugation, Chromatographic & Electrophortic techniques
Description
The analytical methods that are frequently employed in biochemistry are crucial, and mastering them is not only necessary but also vital. This is due to the fact that the solid foundation provided by the methods and experiments upon which the theories are based is the primary source of knowledge on the subject. This course covers a wide range of techniques, their principles, experimental details, etc. These include centrifugation (sub-cellular fractionation, etc.), separation techniques based on hydrodynamic properties, chromatographic and electrophortic techniques, HPLC, purification procedures, ELISA, and other techniques. Use in clinical biochemistry, molecular diagnostics, PCR, RIA, PET, and other clinical diagnostic techniques; also applied in biomedical research. This Course includes: Aqueous solutions , Acids, Bases, buffers systems and pH meter, Colorimetry and UV-VIS absorption spectroscopy Introduction to Hydrodynamic Techniques. Centrifugation: Principles & Methodology Density Gradient centrifugation : Rate Zonal and isopycnic Differential centrifugation for Sub-cellular Fractionation. Gel filtration: Principle, Methodology & Applications Planar Chromatography: Principles and Applications. Ion-exchange chromatography, Affinity chromatography. Gas chromatography, HPLC, Protein Estimation Techniques. Electron Microscopy, TEM and SEM Fluorescence Microscopy : Application to live cell imaging. IHC and IF, Flow cytometry: Theory and concept Flow cytometry:Application in biology and medicine. Light Microscopy and Confocal Microscopy, SDS-PAGE. Western Blotting Techniques, 2D Electrophoresis and DIGE, Principles of Mass spectrometry
Who Should Attend!
- Life science students, chemistry students, Engineering and Technology Students.