Biogenius: Gene Cloning and Expression [Theory & Practice]
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What You Will Learn!
- Understand the foundational principles of recombinant DNA technology and its historical significance.
- Master the ligation process essential for joining DNA fragments in gene cloning.
- Acquire proficiency in performing restriction digestion experiments and manipulating DNA fragments.
- Grasp the transition of plasmids into cloning vectors and the importance of vector selection.
- Examine the pioneering work of Cohen and Boyer in creating the first plasmid vector and how these vectors work.
- Analyze the structure and significance of the pBR322 plasmid in genetic engineering
- Develop effective cloning strategies and troubleshoot common challenges in gene cloning
- Explore the characteristics and applications of PUC series plasmids
- Master the construction and utilization of DNA libraries in lambda phage for gene identification.
- Understand the role and applications of M13 phage vectors in DNA sequencing.
- Explore the features and applications of DNA libraries in COSMIDs for improved gene cloning.
- Learn the principles behind constructing cDNA libraries and their applications in gene expression.
- Master the PCR technique for efficient DNA amplification.
- Understand the principles and applications of TA cloning for simplified gene insertion.
- Gain a comprehensive understanding of the lac operon and its role in gene regulation.
- Explore strategies for modifying the lac operon to suit specific research objectives.
- Identify and comprehend key genetic elements crucial for gene expression.
- Understand the significance of RBS, start and stop codons in translation and protein synthesis.
- Explore different promoters, including P lac and P Tac, and their applications in gene expression.
- Investigate host-specific promoters such as Pbad and Rha Pbad in gene regulation.
- Explore the use of phage promoters in gene expression and their advantages and challenges.
- Understand the two-step system for controlled gene expression and promoter combinations.
- Grasp the role of replication origin in plasmid replication.
- Master the technique of co-expressing genes from two plasmids.
- Differentiate between various types of expression vectors and understand gene insertion principles.
- Evaluate the advantages and limitations of bacterial expression systems.
- Define the objectives and diverse applications of gene expression studies
- Understand the concept of expressing fusion proteins and their applications.
- Explore pull-down assays for studying protein interactions and understand signal peptides' role.
- Master the principles and applications of Topo cloning for simplified gene cloning.
- Identify and characterize expression strains and understand their contribution to efficient gene expression.
Description
This cutting-edge course is meticulously crafted to empower aspiring molecular biologists with the essential knowledge underpinning recombinant DNA technology and genetic manipulation. As a participant, you will embark on a journey that transcends theoretical complexities, providing you with a robust foundation for engaging in practical, real-world applications.
The curriculum spans a wide spectrum of topics, ranging from the fundamental principles of molecular biology to the intricacies of gene cloning techniques. Through a blend of in-depth lectures, interactive discussions, and case studies, you will not only grasp the theoretical intricacies but also cultivate the analytical skills necessary to tackle multifaceted challenges.
Furthermore, this course places a strong emphasis on hands-on experiences, ensuring that you are well-prepared for the dynamic landscape of molecular biology. Laboratories and workshops will offer you the opportunity to apply theoretical knowledge in a practical setting, fostering a seamless transition from theory to application.
As you progress, you will explore advanced topics such as expression systems, recombinant DNA technology, and ethical considerations in genetic engineering. The goal is not only to deepen your understanding but also to equip you with the tools and insights needed to contribute meaningfully to the rapidly evolving field of biotechnology.
By the end of this transformative program, you will emerge not only with a profound theoretical grasp of molecular biology but also with practical expertise, ready to shape the future of biotechnology and molecular research. Join us on this educational odyssey, and become a proficient molecular biologist prepared to make a significant impact in the ever-evolving world of genetic science.
Who Should Attend!
- Entrepreneurs in Biotech Startups
- Biochemistry Graduates
- Undergraduate Biology Students with Research Aspirations
- Healthcare Professionals
- Veterinary Scientists
- Pharmacologists
- Medical Students
- Microbiologists
- Medical Laboratory Technologists
- Neuroscientists
- Botanists
- Bioinformatics Specialists
- Aquatic Biologists
- Food Scientists and Technologists
- Immunologists
- Clinical Researchers
- Biological Anthropologists