What You Will Learn!
- Principles of DNA Cloning
- Identifying the suitable restriction sites on the Cloning vector and on the insert DNA
- How to look for the suitable cloning vector for your experiment
- How to design a primer
Description
The use of bioinformatics and computational biology to study gene cloning has been an auxiliary method used to help implement the DNA cloning in the lab, it facilitates the tools needed to conduct the process efficiently and accurately.
the course provides easily handled and non-time consuming software, it gives you the privilege to dive into the deeps of DNA cloning details, cloning vectors are the key elements for the success of the DNA cloning process as it carries the gene of interest and transfer it to the site of importance. So, in order to conduct the process you have to get the knowledge of the locations of restriction sites and restriction enzymes focusing on the certain locations for the purpose of testing the success of the insertion into the cloning vector.
The cloning vector and the gene of interest must be both cut at the same restriction sites using the same restriction enzymes in order to make sticky ends to facilitate their ligation later on, the cut must be done in the middle of a molecular marker in order to test the success of the process and this can be easily done by knowing not only the locations of the restriction sites but also the locations of molecular markers and locations of the promoters for gene expression later on.
In order to know the exact position of promoter this region must be amplified and in order to do that you have to possess primers, that is why Snap gene software also provides you with tools to design a primer.
Who Should Attend!
- Beginner cloning researchers