What You Will Learn!
- Introduction
- Isolation of RNA
- Isolation of DNA
- Isolation Nucleic acids Applications
- Isolation of DNA and RNA steps
- Diagram support explanation
- Cosmids for further learning
Description
·Introduction:
Step 1: Sample Collection and Protection
Step 2: RNA Preparation
Step 3: Quantitation of Isolated RNA
Step 4: Storage of Isolated RNA
Step 1: Sample Collection and Protection
Finding the most appropriate method of cell or tissue disruption for your specific starting material is important for maximizing the yield and quality of your RNA preparation. During sample disruption for RNA isolation,
Samples stored at 4°C generate intact RNA, even after storage for a month.
Step 2: RNA Preparation
A number of RNA preparation technologies are widely available that can be classified into four general techniques:
organic extraction methods, spin basket formats, magnetic particle methods, and direct lysis methods.
While all can be used to prepare high-quality RNA suitable for a wide variety of analysis techniques, there are several factors to consider in selecting the right purification technology.
Direct Lysis Methods
Direct lysis methods perform sample preparation (not purification) by utilizing lysis buffer formulations that disrupt samples, stabilize nucleic acids, and are compatible with downstream analysis. Typically, a sample is mixed with lysis agent, incubated for some amount of time under specified conditions, and then used directly for downstream analysis. If desired, samples can often be purified from stabilized lysates. By eliminating the need to bind and elute from solid surfaces, direct lysis methods can avoid bias and recovery efficiency effects that may occur when using other purification methods.
Who Should Attend!
- All life science Msc students
- Bsc students
- Bio technology
- Bio chemistry
- Medical student
- Pharmacy
- Pharmacy
