Learn DNA Fingerprinting from the scratch.
Roles of Biotechnology in DNA fingerprinting & Forensics
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What You Will Learn!
- Confirming if two people are related to one another.
- It will take 3 months to fully cover this course.
- It's time consuming and needs a lot of time.
- Rita Curran was violently murdered in her Vermont apartment in 1971 – and the case remained a mystery for decades. But, after 52 years, Curran's family finally
- Defines the roles and responsibilities of a Forensic analyst?
- It will take a whole of 6 months to cover this course fully. The budget will be around $7000
Description
DNA fingerprinting, also called DNA typing, DNA profiling, genetic fingerprinting, genotyping, or identity testing, in genetics, is a method of isolating and identifying variable elements within the base-pair sequence of DNA (deoxyribonucleic acid). The technique was developed in 1984 by British geneticist Alec Jeffreys, after he noticed that certain sequences of highly variable DNA (known as minisatellites), which do not contribute to the functions of genes, are repeated within genes. Jeffreys recognized that each individual has a unique pattern of minisatellites (the only exceptions being multiple individuals from a single zygote, such as identical twins).
The procedure for creating a DNA fingerprint consists of first obtaining a sample of cells, such as skin, hair, or blood cells, which contain DNA. The DNA is extracted from the cells and purified. In Jeffreys’s original approach, which was based on restriction fragment length polymorphism (RFLP) technology, the DNA was then cut at specific points along the strand with proteins known as restriction enzymes. The enzymes produced fragments of varying lengths that were sorted by placing them on a gel and then subjecting the gel to an electric current (electrophoresis):
The fragments underwent autoradiography in which they were exposed to DNA probes—pieces of synthetic DNA that were made radioactive and that bound to the minisatellites. A piece of X-ray film was then exposed to the fragments, and a dark mark was produced at any point where a radioactive probe had become attached.
Who Should Attend!
- Lovers of biotechnology will enjoy this course.
- Learners are curious to know how detectives use forensics to crime related issues.