What You Will Learn!
- Definition of Replication, process of replication in prokaryotes and eukaryotes
- DNA damage and repair system
- Definition of transcription, process of transcription in prokaryotes and eukaryotes
- Post transcriptional modification and drugs which inhibit transcription
- Translation, process of translation in both prokaryotes and eukaryotes.
- Post translational modification
- Regulation of Gene expression, lacoperon, tryoperon
Description
Introduction to the course
Provides information on
Brief introduction on Tutor
Benefits from the course
Topics/Structure of the course
Who can enroll to the course
UNIT 1 : Replication
Central Dogma of biology
Replication of DNA
Semiconservative replication
Complimentary base pairing
Supercoil and DNA topoisomerase
Replication fork
DNA synthesis catalysed by DNA Polymerase
DNA Damage and Repair
Nucleotide Excision Repair
Base Excision Repair
Mismatch Repair system
UNIT 2: Transcription
Central Dogma of Life
what is Transcription
Mechanism Of Transcription
In Prokaryotes
Initiation
elongation
Termination
In Eukaryotes
Initiation is initiated with help of promotor region
TAAT Box and Pribnow Box
Elongation
Enhancers and Silencers
Termination
Post Transcriptional Modification
Addition of Cap and Poly A tail
Splicing Of mRNA
mRNA Editing
Inhibitors of Transcription
Reverse Transcriptase and RNA Degradation.
UNIT 3 : Translation
Genetic Code
Features Of Genetic Code
Codon - Anticodon Pairing
wobble Hypothesis
Link Between Amino Acid And Nucleic Acid
Translation In Prokaryotes
Initiation
Elongation
Termination
How much Energy is consumed during the Process
Translation in Eukaryotes
Initiation with Pre Initiation complex
Association of Ribosomal Subunit
Elongation
Termination using Termination Codons
Release Factors
Post Translational Modification
Trimming
Structural Reorganisation
Modification at N and C Terminal Ends
Covalent Modification
How much Of energy is consumed during the process of Translation
Unit 4: Regulation of Gene Expression
Enhancers and silencers
House keeping and invisible genes
One cistron -one subunit concept
DNA organisation and Gene expression
Covalent Modification of Histones control Gene expression
Regulation of Gene Expression in Prokaryotes :
Lac operon
structure of Lac Operon
Tryptophan regulation in Eukaryotes
Structure of Tryptophan Operon with structural gene
Who Should Attend!
- Students who are in graduation level in the field of biology, molecular biology and Biotechnology
